NASBA – rapid, portable point-of-use testing solutions
Spectrecology has teamed with PureMolecular to develop products for rapid point of use testing based on NASBA technology. Our first product – GrouperChek – is used for discriminating highl value Grouper species from cheaper imposter fish. Detecting mislabeled and fraudulent seafood is a growing need. Our technology can be applied to many applications in food safety, infection detection, species identification, virtually any application where detection of living materials is required.
Call us to discuss your project!
We can develop and manufacture NASBA tests and reagents to meet your application.
Spectrecology 727.230.1607 NASBA@spectrecology.com
NASBA is fast!
NASBA is similar to PCR (polymerase chain reaction) in that the target nucleic acid is replicated or amplified. NASBA is faster than PCR for 2 reasons:
NASBA amplifies mRNA, which occurs as thousands of copies per cell. PCR targets DNA which exists as a single copy per cell
NASBA is isothermal. PCR requires temperature cycling to uncoil the double helix of DNA
NASBA can yield positive results in 10 – 30 minutes compared to ~24 hours for PCR
NASBA targets living cells!
NASBA targets mRNA, present only in living cells and only when genes are being expressed. PCR detects DNA in living and dead cells.
NASBA does not rely on cultures!
Many micro-organisms cannot be cultured, and so are not detected with classical methods. NASBA tests all the microorganisms that are present in the sample
NASBA is quantitative!
Internal controls in our tests allow for excellent quantification
NASBA is easy to use!
Our specialty is making easy to use test kits and instrumentation for point of use by non laboratory professionals.
PureMolecular’s GrouperChek™ assay utilizes real-time nucleic acid sequence-based amplification (NASBA) to amplify a genetic region and molecular beacons for detection. Three enzymes work in concert to achieve exponential RNA amplification without the need for thermal cycling. Reactions are completed in less than 30 minutes, allowing users to perform fast qualitative analysis. Additionally, GrouperChek™ enables the use of crude sample preparation, simplifying the overall testing process. Our testing kits include PureMolecular’s proprietary suite of oligonucleotides and RNA extraction materials, along with highly efficient and robust NASBA reagents.Buy Grouperchek Online!
Point of Use GrouperChek™ Testing Kits
- Detects imposter Grouper
- Performed by the customer
- Identifies Grouper species based on the FDA Seafood List
Result provided in approximately 1 hour
We supply the customer with GrouperCheck™ real-time NASBA testing kits which contain enough reagents and supplies to process up to 8 individual fish samples. The tests are performed on either our AmpliFire™ handheld sensor, or compatible lab-based instrumentation owned by the customer.
$299 per testing kit (reagents for 8 samples — $37.40 /sample)
Detectable grouper Species using GrouperChek NASBA assay
|Scientific Name||Common Name|
|Aethaloperca rogaa||Redmouth Grouper|
|Anyperodon leucogrammicus||Slender Grouper|
|Cephalopholis sonnerati||Tomato Hind|
|Dermatolepis inermis||Marbled Grouper|
|Epinephelus poecilonotus||Dot-dash Grouper|
|Epinephelus acanthistius||Gulf Coney|
|Epinephelus aeneus||White Grouper|
|Epinephelus amblycephalus||Banded Grouper|
|Epinephelus areolatus||Persian Grouper|
|Epinephelus bleekeri||Dusty Tail Grouper|
|Epinephelus chlorostigma||Brownspotted Rockcod|
|Epinephelus coioides||Orange-spotted Grouper|
|Epinephelus cyanopodus||Speckled Blue Grouper|
|Epinephelus diacanthus||Spinycheek Grouper|
|Epinephelus fasciatus||Blacktip Grouper|
|Epinephelus fuscoguttatus||Mottled Grouper|
|Epinephelus guttatus||Red Hind|
|Epinephelus heniochus||Bridled Grouper|
|Epinephelus hexagonatus||Starspotted Grouper|
|Epinephelus lanceolatus||Giant Grouper|
|Epinephelus latifasciatus||Striped Grouper|
|Epinephelus macrospilos||Snubnose Grouper|
|Epinephelus malabaricus||Malabar Grouper|
|Epinephelus marginatus||Dusky Grouper|
|Epinephelus merra||Speckled Dwarf Grouper|
|Epinephelus miliaris||Netfin Grouper|
|Epinephelus morio||Red Grouper|
|Epinephelus morrhua||Comet Grouper|
|Epinephelus multinotatus||White-blotched Grouper|
|Epinephelus mystacinus||Misty Grouper|
|Epinephelus nigritus||Warsaw Grouper|
|Epinephelus niveatus||Snowy Grouper|
|Epinephelus polyphekadion||Camouflage Grouper|
|Epinephelus radiatus||Oblique-banded Grouper|
|Epinephelus rivulatus||Halfmoon Grouper|
|Epinephelus sexfasciatus||Sixbar Grouper|
|Epinephelus striatus||Nassau Grouper|
|Epinephelus tauvina||Greasy Rockcod|
|Epinephelus undulosus||Wavy Lined Grouper|
|Hyporthodus flavolimbatus||Yellowedge Grouper|
|Hyporthodus niphobles||Star-studded grouper|
|Mycteroperca bonaci||Black Grouper|
|Mycteroperca interstitialis||Yellowmouth Grouper|
|Mycteroperca jordani||Gulf Grouper|
|Mycteroperca rubra||Comb Grouper|
|Mycteroperca tigris||Tiger Grouper|
|Mycteroperca venenosa||Yellowfin Grouper|
|Mycteroperca xenarcha||Broomtail Grouper|
The magic starts with careful selection of the sequences of the RNA to be targeted. Sequences that are unique for each target in the set of targets are selected. These can be specific for species, or for strains with particular genes for drug resistance or other expressions. Lysing agents for sample dissolution are designed as well as the molecular beacons to work with the oligo’s. The assays are tested and optimized and appropriate packaging is developed for the anticipated application and the end user.
Our typical applications involve testing for a limited suite of potential species or strains of living materials. Our portable Amplifire NASBA isothermal cycler has room for 8 pcr reaction tubes. The tests can be designed with up to 3 colors of fluorescent labels in a single tube — meaning we can measure up to 24 RNA targets from a single sample.
We have patented technology to detect several noxious microbes using NASBA amplification.
- Enterovirus (human pathogen)
- Norovirus (food-borne pathogen)
- Karenia brevis (Harmful algal blooms)
We are also working with clients on assays in other application areas including authentication, detection, food safety.
The AmpliFire by Douglas Scientific is a portable point of use instrument optimized for isothermal chemistry with the capability to process eight samples simultaneously. With data storage, touch screen interface and easy analysis, the AmpliFire allows you to bring your lab anywhere!Buy Amplfire Online!
Mass: 2.2kg (4.9lbs)
Full Working Load: 2.8Amps
Battery Type: Lithium Ion
Battery Charge Life: 4 to 6 hours
Display: Real time data display with end point outcomes
Transfer: USB 2.0
Fluorescence 3 Channel Excitation / Detection:
- Channel 1 = 495nm / 520nm
- Channel 2 = 535nm / 556nm
- Channel 3 = 590nm / 610nm
Scan Interval: 10 seconds
Reaction Volume: 25µL to 50µL
Reaction Control Temperature: 45C to 80C
Capable of wireless mouse or keyboard connection
Operating: 0C to 40C (32F to 105F)
The Great Plate Anomaly
“The Great Plate Count Anomaly.” About 100 times more cells will be observed microscopically than colonies counted on the Petri dish (Fig. 1). This simple result is one of the most profound puzzles in microbiology, and one of the most significant unsolved questions in biology. — This is a great article by Kim Lewis in the blog Small Things Considered. Click on the link below to read the article.