Spectrecology Raman Systems QEPro Nirquest

Spectrecology Raman SystemReady to Go!

Our Fiber Optic Raman Spectroscopy systems include:

  • High resolution, low noise spectrometer
  • Wavelength stabilized multimode laser
  • Fiber optic Raman probe with built in filters and focusing optics
  • Sample compartment suitable for measuring solids or liquids in vials.
  • Oceanview Software

Raman spectroscopy system components are selected and optimized for one of four laser excitation wavelengths: 532nm, 638nm, 785nm and 1064nm.

532 Raman System

QEPRO-RAMAN-532 Preconfigured QEPRO for 532nm Raman
LASER-532-LAB-FC 532 nm Turnkey, DPSS Laser with >100 mW output power, FC connector, PN I0532SL0100MF
RIP-RPB-532-FC-SMA Raman coupled fiber probe for 532 nm with FC Excitation-SMA Collection
RIP-PA-SH Compact sample holder use with RIP-XXX probes #RAM-PR-SH (KB

638nm Raman System

Raman bundle 638nm
QEPRO-RAMAN-638 QEPRO-RAMAN-638 spectrometer Assembly
LASER-638-LAB-FCA 638 nm Turnkey, Diode Laser with >50 mW output power, FC/APC connector; PN I0638SL0050MA
RIP-RPB-638-SMA-SMA Raman coupled fiber probe for 638 nm with SMA connectors on both fibers
RIP-PA-SH Compact sample holder use with RIP-XXX probes #RAM-PR-SH (KB

785nm Raman System

QEPRO-RAMAN QEPRO Preconfigured for 785nm Raman
LASER-785-LAB-ADJ-FC 785 nm Turnkey, power adjustable, Spectrum Stabilized Multi-Mode laser with > 350mW output power, FC connector, PN I0785MM0350MF
RIP-RPB-785-SMA-FC Raman coupled fiber probe for 785 nm with SMA Excitation -FC Collection; 7.5 mm working distance
RIP-PA-SH Compact sample holder use with RIP-XXX probes #RAM-PR-SH (KB

1064nm Raman System

NIRQuest512-1.9 NIR Spectrometer, 1100-1900 nm, 512-element InGaAs array; includes grating NIR3 (900-1700 nm), CGA-1000 Filter, and SLIT-25
LASER-1064-LAB-ADJ-FC 1064 nm Turnkey, power adjustable, Spectrum Stabilized Multi-Mode laser with >500mW output power, FC connector, PN I1064MM0500MF
RIP-RPB-1064-FC Raman Fiber Coupled Probe for 1064nm with FC connector
RIP-PA-SH Compact sample holder use with RIP-XXX probes #RAM-PR-SH (KB

Our QEPro spectrometer with its exceptional quantum efficiency, cooled back thinned detector, user interchangeable slits and built in shutter is used with the visible wavelength lasers (532, 633, 785nm).

QEPro OEM-Data-Sheet

The 1064nm system uses our NIRQuest512 cooled InGaAs near infrared spectrometer


High-power, Spectrum-stabilized Lasers

Spectrecology frequency stabilized high power Raman Laser 532, 785, 1064

Engineering Specifications LASER-532 Series
Dimensions: 115 mm x 175 mm x 245 mm or(4.5 in x 6.8 in x 9.6 in)
Weight: 1.6 kg (3.4 lbs)
Excitation wavelength: 532 nm
Output power: >50 mW
Fiber connector: SMA 905 or FC
Wavelength stability: +/- 0.1 nm (-20 °C to 55 °C) over temperature range and lifetime
Spectral linewidth: < 0.05 nm (FWHM)
Engineering Specifications LASER-785 Series
Dimensions: 115 mm x 175 mm x 245 mm or(4.5 in x 6.8 in x 9.6 in)
Weight: 1.6 kg (3.4 lbs)
Excitation wavelength: 785 nm
Output power: >350 mW (standard models); variable to >350 mW (adjustable models)
Fiber connector: SMA 905 or FC
Wavelength stability: <3% peak-to-peak in 8 hours
Spectral linewidth: < 0.15 nm (FWHM)


Laser 532 Op Manual

RPB and RPS Raman probes are versatile sampling accessories for lab applications. The probes are available for 532 nm, 785 nm and other excitation wavelengths, with FC and SMA 905 connectors for excitation and collection fibers. RPB probes are anodized aluminum with a stainless steel tip and include a manual safety shutter; RPS probes are stainless steel and include an emission indicator. These are non-immersion probes that are ideal for measurement of solids and surfaces and for measurement through glass and plastics.

Engineering Specifications RPB Probes RPS Probes
Excitation wavelengths: 532 nm and 785 nm (standard) 532 nm and 785 nm (standard)
Spectral range:* 300 – 3900 cm-1  250 – 3900 cm-1 
Laser line blocking: OD 6 OD 8
Sampling head: Anodized aluminum Stainless steel
Probe length: 107 mm 76 mm
Probe diameter: 12.7 mm 12.7 mm
Working distance: 7.5 mm (standard) 5.0 mm (standard)
Fiber configuration: Excitation and collection fibers; 0.22 NA Excitation and collection fibers; 0.22 NA
Fiber length: 1.5 m 5 m
Fiber connectors: FC (standard) and SMA 905 FC (standard) and SMA 905
Built-in safety shutter: Yes No
RPB Probe
Spectrecology Raman Probe OD 8
RPS Probe


The Utility of Raman Spectroscopy

Raman spectroscopy has become a powerful tool for the analysis of materials – in the field, in the lab, and even in clinical settings. The ability to measure spectral fingerprints and compare them to a library of known substances allows identification of pharmaceutical ingredients at the loading dock and explosive materials in the field. In clinical applications, statistical analysis of Raman spectra enables detection of changes in genetic material, proteins and lipids, allowing its use to discriminate between healthy and unhealthy tissue or to detect changes at the cellular level.

Save on preconfigured Raman systems for 532, 638, 785 and 1064 nm wavelengths.

Raman spectroscopy uses nonelastic scattering of laser light from a molecule to probe its molecular structure. Of every million photons bombarding the sample, one lone photon either gains or loses a small amount of energy, corresponding to a vibrational transition within the sample. As this happens again and again, a molecular fingerprint of the sample is gradually built up – one that can rival an FTIR spectrum, yet without the inconvenience of elaborate sample preparation or water interference. Even better, since the incident laser light is not being directly absorbed, it does not require a specific excitation laser. At least in theory

Raman Effect

Raman scattering from a molecule results in light of a slightly longer or shorter wavelength than the excitation laser, with the energy difference corresponding to a vibrational energy level transition within the molecule.

The Importance of Wavelength

Though any wavelength can be used to stimulate the Raman effect given enough incident intensity, some wavelengths are better than others, particularly for certain sample types. Ocean Optics offers a range of bundled modular fiber optic Raman spectroscopy systems to suit a wide range of applications, and the guidance to help you select the right one for your application.

The probability of Raman scattering decreases rapidly as the excitation wavelength of the laser increases, scaling as 1/λ4. This means that increasing your laser wavelength merely from 532 nm to 638 nm will cost you half of your signal, and going to 785 nm drops it to nearly one-fifth! Though this might steer one to the use of shorter laser wavelengths, there is autofluorescence to be considered. Organic and biological samples tend to fluoresce when exposed to high laser intensities, creating a broad background that can obscure the Raman signal, which may degrade signal to noise and make Raman peaks difficult to resolve even when observed. Autofluorescence is strongest when the excitation laser energy corresponds to an electronic transition within the sample, and is typically greatest at visible laser wavelengths for organic materials. Polymers, pharmaceuticals, many synthetic materials and dyes are particularly subject to this effect.

For this reason, organic samples are often studied using red or NIR excitation wavelengths, where the benefits of reduced autofluorescence background easily compensate for the anticipated need for a longer acquisition time or increased laser intensity. In fact, autofluorescence can be almost completely eliminated in most samples by using 1064 nm laser excitation, as this energy is too low to excite an electronic transition in most materials. It can also be avoided by working at ultraviolet excitation wavelengths (200-250 nm), as the entire Raman spectrum of interest can be captured prior to the onset of autofluorescence at ~300 nm.

Benefits and Applications by Wavelength

Ocean Optics offers a variety of modular and turnkey solutions for Raman spectroscopy from the UV to NIR, including bundled systems for 532, 638, 785 and 1064 nm. Let’s consider the benefits and applications typically studied by wavelength.

1064 nm

This excitation wavelength has been gaining in popularity in recent years, due in large part to the minimal fluorescence generated, particularly for pigment-rich tissues and materials that can be troublesome even at shorter NIR wavelengths. Though the Raman signal is much weaker (6% of what would be predicted for 532 nm), the near-absence of fluorescence permits spectra to be obtained with a reasonable signal to noise ratio. Though the Fourier transform method (FT-Raman) is often used when working at 1064 nm excitation, sensitive diode-array spectrometers such as the NIRQuest can also offer adequate sensitivity and resolution for many applications. Care should be taken to avoid overheating of biological samples due to the longer wavelength illumination.

Applications: polymers, oils, dyes, plant biomass, biological tissue, edible oils, petrochemicals


785 nm

The most popular Raman excitation wavelength is 785 nm. Raman 785 nm systems yield excellent quality Raman spectra for most chemicals, with limited interference from fluorescence. These systems also offer very good spectral resolution, making it perhaps the preferred wavelength choice for general Raman spectroscopy of chemicals and organic materials.

Applications: polymers, biological tissue, active pharmaceutical ingredients (APIs), art pigment identification, edible oils, petrochemicals, foods, explosives, sorting of dark plastics, through-bottle inspection, narcotics, SERS

Final melamine spectrum.xlsx


638 nm

Raman spectroscopy at 638 nm offers many of the same benefits as working at 785 nm, balancing signal level with fluorescence and offering very good resolution. This wavelength is often used for most biomedical applications, which need to balance the risk of sample damage with fluorescence generation. We have also found 638 nm to be an extremely versatile wavelength for generating high-quality SERS data for a wide range of analytes, from trace detection of explosives to pesticides, fungicides, and more.

Applications: biomedical instrumentation, corrosion, pesticides, fungicides, SERS




In Surface Enhanced Raman Spectroscopy (SERS), analytes are adsorbed on to a silver or gold surface prior to analysis, boosting the Raman signal intensity by millions of times. The use of solid state substrates for SERS allows ppb-level detection of chemical and biological materials in the field, as well as in pharmaceuticals, explosives and tags for anti-counterfeiting.

Most SERS substrates are fabricated using expensive lithography techniques and are not reusable, making cost a deterrent for mainstream applications. Ocean Optics inkjet-printed SERS substrates offer better performance at a fraction of the price, with peak intensity ratios repeatable to within 5%. Using SERS substrates is easy with our quick start guide.

532 nm

This wavelength is the workhorse for inorganic materials, offering maximum signal for samples that do not suffer from autofluorescence. Often used for the study of carbon nanotubes, fullerenes and other carbon materials to avoid sample burning, 532 nm excitation is also good for resonance Raman experiments.

Applications: semiconductor materials, catalysts, polymers, minerals, carbon nanotubes and nanowires, temperature measurement, plasmon and superconducting gap excitation studies, nanowire composition, silicon crystallinity in solar cell manufacture, gemstone analysis and authentication

Source: http://www.spectroscopyeurope.com/articles/55-articles/3290-surface-enhanced-raman-scattering-sers-spectroscopy-identifies-fraudulent-uses-of-fuels

A 90:10 mixture of unmarked diesel-kerosene (blue trace) is easily distinguished from a mixture in which the kerosene diluting the diesel contains a marker (red trace).
Source: http://www.spectroscopyeurope.com/articles/55-articles/3290-surface-enhanced-raman-scattering-sers-spectroscopy-identifies-fraudulent-uses-of-fuels