Reflection Measurements

% Reflection or reflectance is measured as the ratio of light reflected from a sample to light reflected from a reference material. It is usually expressed in a percentage scale (%R). Fixtures and probes are used to define the illumination and reflected light angles, allowing for the measurement of specular (mirror like) and/or diffuse reflection. In a typical setup, the light source and spectrometer are connected by optical fibers to the reflection measurement fixture. In the illustration the fixture is a fiber optic probe held at 45 degrees to the sample. The system can be purchased as a kit.
Buy a Flame reflection System online!

SE-REF-KIT 45/45/90
This is an aluminum fixture for looking at reflection from flat surfaces. There are 3 ports to allow for illumination at 45, 90 and 135 degrees. The ports accommodate screw in fiber optic collimators (2 are included with the kit) or black plugs to block light. An additional adapter will hold a 1/4″ OD reflection probe. The ports fit our SE-112689 collimator with quartz, sapphire or AR coated lenses. The ports also accommodate the 74-UV and 74-ACR style lenses.
Model Number | SE-REF-KIT |
Materials | Fused Silica, Aluminum (black anodized) |
RoHS Compliant | Yes |
Max. Bake Temperature | 80 °C |
Max. Operating Temperature | 80 °C |
Max. Vacuum Level | Air Service |
Length / Height | 2.30 Inch |
ID | 0.25 Inch |
OD | 1.00 Inch |
Operating Wavelength | 250~2000 nm |
R400-7 and R-600-7-UV-SWIR Fiber Optic reflection Probes
The 6 around 1 fiber reflection probe is multi-purpose. It can be used to measure specular or diffuse reflection from a surface, diffuse reflection from liquids, tissue and other translucent materials and fluorescence.
Probes are available in any size fiber, but the R400-7-UV-VIS (or VIS-NIR) or our new R600-UV-SWIR probes are our most popular. They have a 3″ ferrule, 1/4″ OD and 2m long fibers that are bifurcated at the halfway point. These probes can be used with holders such as the SE-REF above, or the RPH-1, to hold it at 45 or 90 degrees. A white (diffuse) or specular reflection standard and a light source completes the setup.
Fiber types include XSR for UV (extreme solarization resistance, 190-900nm), VIS-NIR (350-2200nm) and UV-SWIR (200-2200nm)
Buy UV XSR Reflection Probes online!Buy VIS-NIR Reflection probes online!
Buy UV-SWIR Reflection probes online!
The SE-112802 30 degree reflection adapter provides a large area flat quartz window to accommodate measuring powders, or soft surfaces that must be pressed into a flat surface. This provides essentially “dark field” reflection spectra by eliminating specular reflections in the measurement plane.

Buy large area 30 degree reflection probe attachment online!
Reflection Probe

Engineering Specifications | Premium-grade Reflection Probes |
Probe ferrule material: | Stainless steel |
Probe ferrule length: | 76.2 mm (3″) |
Probe ferrule diameter: | 6.35 mm (1/4″) unless otherwise noted (3.175 mm / 1/8″ for QR600 probes) |
Probe fiber bundle: | 6 illumination fibers around 1 read fiber |
Numerical aperture: | 0.22 ± 0.02 (equivalent to an acceptance angle of 24.8° in air) |
Jacket: | Standard fibers: Silicone-coated steel monocoil with a Nomex braid for superior strain relief and protection |
“-BX” fibers: Stainless steel BX | |
Connectors: | Precision SMA 905 with knurled ferrule |
Length: | 2 m |
Breakout: | Midway point of assembly at 1 meter |
Fiber core size: | 200 – 600 μm (see product variations table for options) |
Wavelength range: | XSR, SR, UV/VIS, VIS/NIR (see product variations table for options) |
Fiber buffer: | See Fiber Bend Radius and Mechanical Specifications for details |
Bend radius: | See Fiber Bend Radius and Mechanical Specifications for details |
Operating temperature: | -20 °C to 80 °C |
Probe attachment
Model Number | SE-112802 |
Materials | Fused Silica, Aluminum (black anodized) |
RoHS Compliant | Yes |
Max. Bake Temperature | 80 °C |
Max. Operating Temperature | 80 °C |
Max. Vacuum Level | Air Service |
Length / Height | 2.30 Inch |
ID | 0.25 Inch |
OD | 1.00 Inch |
Operating Wavelength | 250~2000 nm |
The spot size for reflection off a surface, and the active volume for fluorescence or back scatter vary with distance. Initially the signal increases as the overlap region grows. Then the signal decreases as the distance to the sample increases.


Integrating Spheres

Integrating spheres are used to acquire reflection spectra from 180 degrees. The typical setup is to have a collimated viewing angle of ~8 degrees from normal and diffuse illumination from a baffled light source in the sphere. A removable light trap at the opposite 8 degree angle can be used to eliminate specular reflection. ISP-R series fiber optic integrating spheres couple to Ocean Optics spectrometers to measure the total integrated reflectance of surfaces placed against the sphere’s sample port. ISP-R integrating spheres are well-suited for measurement of opaque or highly directional samples. The spheres are available in 6 mm and 8 mm sample port diameters and gloss-trap options are available that allow you to measure the specular component of the total integrated reflection. Versatile – adaptable for diffuse or specular measurements High reflectivity – PTFE sphere coating is >98% reflective from 400-1500 nm Multiple options – available with sample port diameters of 6 mm and 8 mm
Buy Integrating Spheres Online
Engineering Specifications | ISP-30-6-R | ISP-50-8-R | ISP-50-8-R-GT | ISP-80-8-R |
Dimensions: | 59 mm diameter x 58 mm height | 80 mm diameter x 78 mm height | 80 mm diameter x 78 mm height | 107 mm diameter x 117 mm height |
Weight: | 330 g | 730 g | 743 g | 1,650 g |
Spectral range: | 200-2500 nm | 200-2500 nm | 200-2500 nm | 200-2500 nm |
Sphere diameter: | 30 mm | 50 mm | 50 mm | 80 mm |
Sample port diameter: | 6 mm | 8 mm | 8 mm | 8 mm |
Sphere coating: | PTFE | PTFE | PTFE | PTFE |
PTFE reflectivity: | >98% (400-1500 nm)>95% (250-2500 nm) | >98% (400-1500 nm)>95% (250-2500 nm) | >98% (400-1500 nm)>95% (250-2500 nm) | >98% (400-1500 nm)>95% (250-2500 nm) |
Reflectance measurements: | Specular and diffuse | Specular and diffuse | Diffuse or specular and diffuse | Specular and diffuse |
Gloss trap: | No | No | Yes | No |
Connector: | SMA 905 | SMA 905 | SMA 905 | SMA 905 |

Stage-RTL

Buy a STAGE-RTL online!
Engineering Specifications | STAGE-RTL-T |
Base dimensions: | 206.3 mm diameter |
Sample area dimensions: | 152.4 mm diameter |
Weight: | 4.5 kg |
Rail height: | Adjustable to 400 mm |
Components: | Sample plate, rail, adapter, collimating lenses (2) and light trap |
Collimating lens wavelength: | 200-2000 nm |
Stage material: | Anodized aluminum |
Integrating sphere adapter (optional): | Item ISP-RTL-ADP, for attaching 30 mm or 50 mm ISP-series spheres |
Reflection Standards
Diffuse Standards for Reflectance Measurements
- Diffusing materials – choose from PTFE or Spectralon standards
- High reflectivity over broad range – >95% reflectivity from 250-2200 nm
- More options – additional Spectralon reflectance standards and targets also available

By WS-1 White Standard online!
Buy Spectralon White Standard online!
Engineering Specifications | WS-1 | WS-1-SL |
Dimensions: | 38 mm diameter (housing) | 32 mm OD, 10 mm thick (tile) |
Weight: | 30 g | 30 g |
Spectral range: | 250-2000 nm | 250-2500 nm |
Diffusing material: | PTFE | Spectralon |
Housing material: | Aluminum | Delrin |
Reflectivity: | >98% (250-1500 nm)>95% (250-2200 nm) | 99% (400-1500 nm)>96% (250-2000 nm) |

Actual reflectivity values for the standards are available as an ASCII file. Spectrasuite and Oceanview can use that file to correct for deviations from 100%. NIST traceable versions of the ehite standards are also available.



Buy Specular High Reflectivity Standard online!
Buy Specular Low Reflectivity Standard online!
Engineering Specifications | STAN-SSH | STAN-SSH-NIST | STAN-SSL |
Dimensions (substrate): | 31.75 mm OD x 6.35 mm height | 31.75 mm OD x 6.35 mm height | 31.75 mm OD x 6.35 mm height |
Dimensions (housing): | 38 mm OD x 19 mm height | 38 mm OD x 19 mm height | 38 mm OD x 19 mm height |
Weight: | 40 g | 40 g | 40 g |
Reflectance material: | Al mirror on fused silica substrate | Al mirror on fused silica substrate | Schott ND9 glass |
Reflectivity: | ~87%-93% (200-1000 nm) | ~87%-93% (200-1000 nm) | ~5% (200-950 nm) |
~93%-98% (1000-2500 nm) | ~93%-98% (1000-2500 nm) | ~4% (950-2500 nm) |